MegaCD40L is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30/adiponectin. This construct very effectively simulates the natural membrane-assisted aggreggation of CD40L in vivo. It provides a simple and equally potent alternative to [CD40L + enhancer] combinations.
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Graphic: MegaCD40L - two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30/adiponectin
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Figure: MegaCD40L, Soluble (human) (rec.) (Prod. No. ALX-522-110) does not need an enhancer to induce B cell activation.
Method: PBL cells were incubated in 96-well plates (2x105 cells/well in 100祃 RPMI supplemented with 10% FCS) for 24 hours at 37癈 with the indicated concentration of MegaCD40L, Soluble (human) (rec.) or CD40L; Soluble (human) (rec.) (Prod. No. ALX-522-015) in the presence and absence of 1礸/ml enhancer (Prod. No. ALX-804-034). Cells were washed with PBS and stained with 2 祃 each CD86-PE and CD19-FITC in 50 祃 FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) for 20 min. at 4癈 in the dark. After two washes in FACS buffer, samples were then analyzed by flow cytometry.

Lit. Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex: N. Holler, et al.; Mol. Cell. Biol. 23, 1428 (2003)

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Related products:
CD40 and CD40L products from ALEXIS