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蛋白分析
 
来源: leeyond 加入时间: 2006-09-30 08:43:55 浏览次数:2150
蛋白分析

Cambrex Bio Science Rockland, Inc

Protein Analysis

-----New! Versalinx?Protein Sample Preparation Tools

50602 Experimenter's Kit

50593 Versalinx Amine Modifying Reagent,10 mg

50594 Versalinx Carbohydrate Modifying Reagent,10 mg

50595 Versalinx Disulfide Forming Reagent,10 mg

50596 Versalinx Sulfhydral Alkylating Reagent,10mg

50592 Versalinx SHA-Agarose Chromotography Medium,5ml

描述: Versalinx Protein Sample Preparation Tools employ a unique small molecule, chemical affinity technology.

The basis for this system is the specific interaction between two non-biological molecules, phenyl(di)boronic acid (P(D)BA) and salicylhydroxamic acid (SHA). Under conditions typical for bioseparation, Versalinx complex formation is robust and highly specific and enables the immobilization of P(D)BA conjugated biospecific ligands to SHA modified Agarose facilitating affinity separations.

Features:

>>>Easy-to-use ?Standard coupling reaction conditions.

>>>Reversible ligand linkage under mild conditions ?Enables recovery of precious capture ligand or ligand-target complexes.

>>>Small molecule based chemistry ?High retention of biological activity. Low non-specific binding, even for complex samples such as cell lysates.

>>>Highly specific interaction between SHA and PDBA ?No need to purify sample.

Effortless Implementation:

>>>Quick and simple ?P(D)BA conjugate your capture ligand, bind ligand to column, capture and recover target protein.

>>>P(D)BA modifi cation reagents ?Select site and degree of ligand modification.

>>>SHA-modified Agarose Chromatography Media ?Universal: binds any PDBA-capture ligand.

Now Available - Experimenter's Kit

>>>Kit includes 5 mg of each Modifying Reagent

>>>and 5 ml of SHA Chromotography Medium

--New!!---Transport Protein Delivery Reagent

50568 Transport Protein Delivery Reagent,400 ug

描述: Transport Protein Delivery Reagent is a revolutionary new tool for non-cytotoxic delivery of biologically active cargo molecules (proteins, peptides, or small molecules) directly into live cells. Transport Reagent allows you to quickly and easily study cell and protein function, while avoiding the problems of DNA transfections (promoter interference, variable levels of transfection). This newly discovered peptide (patent pending) translocates itself as well as attached cargo molecules into cultured cells when added to the culture medium. Transport Reagent-Cargo Complex delivery is very efficient (up to 100% of cultured primary cells or cell lines) and allows rapid analysis of the unharmed transduced cells.

Transport Reagent delivers:

>>>Efficient Transfer ?Deliver biologically active proteins, peptides, and other macromolecules in up to 100% of cells.

>>>Speed ?Deliver macromolecules into cells in less than 1-2 hours.

>>>Flexibility ?Transfect a wide variety of cell types including primary cells.

>>>Non-cytotoxicity - Assay live cells and ensure that the measured effect is due to your cargo, not due to the transfection method.

>>>Ease of use ?Simply mix Transport Reagent with your protein and apply to cells.

Several cell lines and primary cells have been successfully transfected with Transport Reagent, including:

Human Bronchial Epithelial Cells

>>>Human Epidermal Keratinocytes

>>>Aortic Smooth Muscle Cells

>>>HUVEC

>>>Human Dermal Fibroblasts

>>>NIH3T3

>>>293

>>>C2C12

The conjugation of Transport Reagent and a cargo molecule occurs through the 2-pyridyldithio group on the reagent which forms a disulfide bond with any free thiol (cysteine) situated on the cargo molecule. Coupling of Transport Reagent with cargo molecules is a simple process ?simply incubate appropriate amounts of each component at room temperature for 15-30 minutes, dilute with culture medium and apply to culturing cells.

Transport Reagent is supplied as a lyophilized powder, which can be reconstituted with water, aliquoted and stored frozen. 400 ug of Transport Reagent is sufficient for 40-80 reactions in a 6-well or 35-mm dish, or 700-1,400 reactions in a 96-well plate. A Beta-Galactosidase control is supplied to allow testing and optimization of your experimental parameters.

-----Protein Electrophoresis

Part Code Description Size

50010 SeaKem ME Agarose 125 g

50011 SeaKem ME Agarose 25 g

50014 SeaKem ME Agarose 500 g

50020 SeaKem HE Agarose 125 g

50021 SeaKem HE Agarose 25 g

50030 SeaKem HEEO Agarose 125 g

50031 SeaKem HEEO Agarose 25 g

50040 SeaKem HGT Agarose 125 g

50041 SeaKem HGT Agarose 25 g

50050 SeaKem HGT-P Agarose 125 g

50202 IsoGel Agarose 25 g

50540 SYPRO Stain Photo Filter (3 in square) 1 ea

50542 SYPRO Red Gel Stain 10 X 50 ul

50543 SYPRO Red Gel Stain 500 ul

50547 ProSieve Protein Marker 500 ul

50550 ProSieve Color Ptn Marker 500 ul

50552 ProSieve Color Ptn Marker 100 ul

50556 SYPRO Tangerine Gel Stain 5,000X (500 ul)

50562 SYPRO Ruby Gel Stain 1 L

50563 SYPRO Ruby Gel Stain 5 L

50564 SYPRO Ruby Gel Stain 200 ml

50565 SYPRO Ruby Blot Stain 200 ml

50617 ProSieve 50 Gel Solution 125 ml

50618 ProSieve 50 Gel Solution 250 ml

50918 Duramide monomer 750 ml

50928 Duramide monomer 100 ml

56010 IsoGel Agarose IEF Plate Accessory Bulk Pack

100 each wicks and blotting paper

56014 IsoGel Agarose IEF Plate Accessory Pack Sufficient for 6 plates

56015 IsoGel Agarose IEF Plates pH 3-10

58501 PAGEr Gold Gels 7.5% 12 well, 9X10 cm, 10 gels

58502 PAGEr Gold Gels 10% 12 well, 9X10 cm, 10 gels

58503 PAGEr Gold Gels 12% 12 well, 9X10 cm, 10 gels

58504 PAGEr Gold Gels 15% 12 well, 9X10 cm, 10 gels

58505 PAGEr Gold Gels 4-20% 12 well, 9X10 cm, 10 gels

58506 PAGEr Gold Gels 10-20% 12 well, 9X10 cm, 10 gels

58507 PAGEr Gold Gels 7.5% 10 well, 9X10 cm, 10 gels

58508 PAGEr Gold Gels 10% 10 well, 9X10 cm, 10 gels

58509 PAGEr Gold Gels 12% 10 well, 9X10 cm, 10 gels

58510 PAGEr Gold Gels 15% 10 well, 9X10 cm, 10 gels

58511 PAGEr Gold Gels 4-20% 10 well, 9X10 cm, 10 gels

58512 PAGEr Gold Gels 10-20% 10 well, 9X10 cm, 10 gels

58513 PAGEr Gold Gels 7.5% 16 well, 9X10 cm, 10 gels

58514 PAGEr Gold Gels 10% 16 well, 9X10 cm, 10 gels

58515 PAGEr Gold Gels 12% 16 well, 9X10 cm, 10 gels

58516 PAGEr Gold Gels 15% 16 well, 9X10 cm, 10 gels

58517 PAGEr Gold Gels 4-20% 16 well, 9X10 cm, 10 gels

58518 PAGEr Gold Gels 10-20% 16 well, 9X10 cm, 10 gels

58519 PAGEr Gold Gels 8-16% 10 well, 9X10 cm, 10 gels

58520 PAGEr Gold Gels 4-12% 10 well, 9X10 cm, 10 gels

58521 PAGEr Gold Gels 4-12% 12 well, 9X10 cm, 10 gels

58522 PAGEr Gold Gels 8-16% 12 well, 9X10 cm, 10 gels

58523 PAGEr Gold Gels 8-16% 16 well, 9X10 cm, 10 gels

58524 PAGEr Gold Gels 4-12% 16 well, 9X10 cm, 10 gels

58545 PAGEr Gold, 17 well 9X10cm, 10 gels

58551 PAGEr Gold Gels 4-20% 9 well, 9X10 cm, 10 gels

58562 PAGEr Gold Gels 8-16% 9 well, 9X10 cm, 10 gels

58601 PAGEr Gold Gels 7.5% 12 well, 9X10cm, 10 gels

58602 PAGEr Duramide Gels 10% 12 well, 9X10cm, 10 gels

58603 PAGEr Duramide Gels 12% 12 well, 9X10cm, 10 gels

58605 PAGEr Duramide Gels 4-20% 12 well, 9X10cm, 10 gels

58606 PAGEr Duramide Gel 10-20% 12 well, 9X10cm, 10 gels

58607 PAGEr Gold Gels 7.5% 10 well, 9X10cm, 10 gels

58608 PAGEr Duramide Gels 10% 10 well, 9X10cm, 10 gels

58609 PAGEr Duramide Gels 12% 10 well, 9X10cm, 10 gels

58610 PAGEr Duramide Gels 15% 10 well, 9X10cm, 10 gels

58611 PAGEr Duramide Gels 4-20% 10 well, 9X10cm, 10 gels

58619 PAGEr Duramide Gels 8-16% 10 well, 9X10cm, 10 gels

58642 PAGEr Duramide, 10% 17 well 9X10cm, 10 gels

58645 PAGEr Duramide, 4-20% 17 well 9X10cm, 10 gels

58646 PAGEr Duramide, 10-20% 17 well 9X10cm, 10 gels

58648 PAGEr Duramide Gels 10% 9 well, 9X10cm, 10 gels

58649 PAGEr Duramide Gels 12% 9 well, 9X10cm, 10 gels

58651 PAGEr Duramide Gels 4-20% 9 well, 9X10cm, 10 gels

58652 PAGEr Duramide Gel 10-20% 9 well, 9X10cm, 10 gels

59501 PAGEr Gold Gels 7.5% 12 well, 10X10cm, 10 gels

59502 PAGEr Gold Gels 10% 12 well, 10X10cm, 10 gels

59503 PAGEr Gold Gels 12% 12 well, 10X10cm, 10 gels

59504 PAGEr Gold Gels 15% 12 well, 10X10cm, 10 gels

59505 PAGEr Gold Gels 4-20% 12 well, 10X10cm, 10 gels

59506 PAGEr Gold Gels 10-20% 12 well, 10X10cm, 10 gels

59507 PAGEr Gold Gels 7.5% 10 well, 10X10cm, 10 gels

59508 PAGEr Gold Gels 10% 10 well, 10X10cm, 10 gels

59509 PAGEr Gold Gels 12% 10 well, 10X10cm, 10 gels

59510 PAGEr Gold Gels 15% 10 well, 10X10cm, 10 gels

59511 PAGEr Gold Gels 4-20% 10 well, 10X10cm, 10 gels

59512 PAGEr Gold Gels 10-20% 10 well, 10X10cm, 10 gels

59513 PAGEr Gold Gels 7.5% 16 well, 10X10cm, 10 gels

59514 PAGEr Gold Gels 10% 16 well, 10X10cm, 10 gels

59515 PAGEr Gold Gels 12% 16 well, 10X10cm, 10 gels

59516 PAGEr Gold Gels 15% 16 well, 10X10cm, 10 gels

59517 PAGEr Gold Gels 4-20% 16 well, 10X10cm, 10 gels

59518 PAGEr Gold Gels 10-20% 16 well, 10X10cm, 10 gels

59519 PAGEr Gold Gels 8-16% 10 well, 10X10cm, 10 gels

59520 PAGEr Gold Gels 4-12% 10 well, 10X10cm, 10 gels

59521 PAGEr Gold Gels 8-16% 12 well, 10X10cm, 10 gels

59522 PAGEr Gold Gels 4-12% 12 well, 10X10cm, 10 gels

59523 PAGEr Gold Gels 8-16% 16 well, 10X10cm, 10 gels

59524 PAGEr Gold Gels 4-12% 16 well, 10X10cm, 10 gels

59544 PAGEr Gold Gels 15% 17 well, 10X10cm, 10 gels

59545 PAGEr Gold Gels 4-20% 17 well, 10X10cm, 10 gels

59551 PAGEr Gold Gels 4-20% 9 well, 10X10cm, 10 gels

59557 PAGEr Gold Gels 4-20% 2D well, 10X10cm, 10 gels

59560 PAGEr Gold Gels 8-16% 17 well, 10X10cm, 10 gels

59601 PAGEr Gold Gels 7.5% 12 well, 10X10cm, 10 gels

59602 PAGEr Duramide Gels 10% 12 well, 10X10cm, 10 gels

59603 PAGEr Duramide Gels 12% 12 well, 10X10cm, 10 gels

59605 PAGEr Duramide Gels 4-20% 12 well, 10X10cm, 10 gels

59606 PAGEr Duramide Gel 10-20% 12 well, 10X10cm, 10 gels

59607 PAGEr Gold Gels 7.5% 10 well, 10X10cm, 10 gels

59608 PAGEr Duramide Gels 10% 10 well, 10X10cm, 10 gels

59609 PAGEr Duramide Gels 12% 10 well, 10X10cm, 10 gels

59610 PAGEr Duramide Gels 15% 10 well, 10X10cm, 10 gels

59611 PAGEr Duramide Gels 4-20% 10 well, 10X10cm, 10 gels

59619 PAGEr Duramide Gels 8-16% 10 well, 10X10cm, 10 gels

59620 PAGEr Duramide Gels 4-12% 10 well, 10X10cm, 10 gels

59622 PAGEr Duramide Gels 4-12% 12 well, 10X10cm, 10 gels

59642 PAGEr Duramide Gels 10% 17 well, 10X10cm, 10 gels

59643 PAGEr Duramide Gels 12% 17 well, 10X10cm, 10 gels

59645 PAGEr Duramide Gels 4-20% 17 well, 10X10cm, 10 gels

59646 PAGEr Duramide Gel 10-20% 17 well, 10X10cm, 10 gels

59648 PAGEr Duramide Gels 10% 9 well, 10X10cm, 10 gels

59649 PAGEr Duramide Gels 12% 9 well, 10X10cm, 10 gels

59651 PAGEr Duramide Gels 4-20% 9 well, 10X10cm, 10 gels

59652 PAGEr Duramide Gel 10-20% 9 well, 10X10cm, 10 gels

59654 PAGEr Duramide Gels 10% 2D well, 10X10cm, 10 gels

59655 PAGEr Duramide Gels 12% 2D well, 10X10cm, 10 gels

59657 PAGEr Duramide Gels 4-20% 2D well, 10X10cm, 10 gels

59658 PAGEr Duramide Gel 10-20% 2D well, 10X10cm, 10 gels

59659 PAGEr Duramide Gels 4-12% 17 well, 10X10cm, 10 gels

59661 PAGEr Duramide Gels 4-12% 9 well, 10X10cm, 10 gels

59663 PAGEr Duramide Gels 4-12% 2D well, 10X10cm, 10 gels

59905 PAGEr Gel Chamber Electrophoresis tank

59906 PAGEr Gel Blot Module Blot module and 8 sponge pads

59907 PAGEr Gel Chamber and Blot Module Kit Tank, blot module and 8 sponge pads

54711 GelBond PAG Film 138mm X 158mm, 50 sheets

54723 GelBond PAG Film 160mm X 180mm, 50 sheets

54727 GelBond PAG Film 124mm X 258mm, 50 sheets

54729 GelBond PAG Film 220mm X 165mm, 50 sheets

54731 GelBond PAG Film 199mm X 264mm, 50 sheets

54733 GelBond PAG Film 203mm X 260mm, 50 sheets

54735 GelBond PAG Film 195mm X 370mm, 50 sheets

54746 GelBond PAG Film 350mm X 430mm, 10 sheets

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